Document 0494
 DOCN  M9480494
 TI    Specificity and sequence requirements for interactions between various
       retroviral Gag proteins.
 DT    9410
 AU    Franke EK; Yuan HE; Bossolt KL; Goff SP; Luban J; Department of
       Medicine, Columbia University, College of; Physicians and Surgeons, New
       York, New York 10032.
 SO    J Virol. 1994 Aug;68(8):5300-5. Unique Identifier : AIDSLINE
       MED/94309202
 AB    We previously established a genetic assay for retroviral Gag polyprotein
       multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S.
       P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to
       demonstrate homomeric interactions between Gag polyproteins encoded by
       six different retroviruses. Of the Gag polyproteins tested, only those
       encoded by closely related retroviruses formed heteromultimers. To
       determine the primary sequence requirements for human immunodeficiency
       virus type 1 Gag polyprotein multimerization, we studied the effects on
       multimerization of deletion and linker insertion mutations. Sequences
       necessary for this process were located between the C-terminal one-third
       of the capsid domain and the C terminus of the nucleocapsid domain.
 DE    Animal  Base Sequence  DNA, Viral  Gene Products,
       gag/GENETICS/*METABOLISM  Genetic Techniques  Human  Molecular Sequence
       Data  Mutagenesis, Insertional  Retroviridae/GENETICS/*METABOLISM
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).